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KMID : 0613820070170050699
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Journal of Life Science
2007 Volume.17 No. 5 p.699 ~ p.705
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Genetic Relationship and Characteristics Using Microsatellite DNA Loci in Horse Breeds
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Cho Gil-Jae
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Abstract
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The present study was conducted to investigate the genetic characteristic and to establish the parentage verification system of the Korean native horse (KNH). A total number of 192 horses from six horse breeds including the KNH were genotyped using 17 microsatellite loci. This method consisted of multiplexing PCR procedure. The number of alleles per locus varied from 5 to 10 with a mean value of 7.35 in KNH. The expected heterozygosity and observed heterozygosity were ranged from 0.387 to 0.841 (mean 0.702) and from 0.429 to 0.905 (mean 0.703), respectively. The total exclusion probability of 17 microsatellite loci was 0.9999. Of the 17 markers, AHT4, AHT5, CA425, HMS2, HMS3, HTG10, LEX3 and VHL20 marker have relatively high PIC value (＞0.7). This study found that there were specific alleles, P allele at AHT5, Q allele and R allele at ASB23, H allele at CA425, S allele at HMS3, J allele at HTG10 and J allele at LEX3 marker in KNH when compared with other horse populations. Also, the results showed two distinct clusters: the Korean native horse cluster (Korean native horse, Mongolian horse), and the European cluster (Jeju racing horse, Thoroughbred horse). These results present basic information for detecting the genetic markers of the KNH, and has high potential for parentage verification and individual identification of the KNH.
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KEYWORD
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Genetic characteristic, Korean native horse, microsatellite marker, specific allele
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